ddPCR Copy Number Kits

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The ddPCR SMN1 and SMN2 Copy Number Determination Kits are designed to determine the copy number of the SMN1 and SMN2 genes respectively. The kit contains a 20x ddPCR Copy Number Determination Assay, positive controls, and ddPCR Supermix for Probes (No dUTP).

Combined with Bio-Rad’s QX100, QX200, or QX200 AutoDG Droplet Digital PCR (ddPCR) System, the ddPCR SMN1 and SMN2 Kits allow you to:

• Achieve superior performance with accurate and reproducible copy number calls for the gene of interest
• Perform high-throughput screening by processing several hundred samples in a single day
• Benefit from economical cost per sample

Sample Data

Detection of SMN1 copy number. A 2-D fluorescence amplitude plot shows the wells of a sample duplexed with the SMN1 and reference assays. The black cluster on the plot represents the negative droplets, the green cluster represents the droplets that are positive for the reference only, the blue cluster within the red circle represents the droplets that are present for SMN1 only, and the orange cluster represents the droplets that are positive for both SMN1 and the reference target.

Accurate and reproducible copy number calls for SMN1. Copy number plot shows positive controls, ranging from zero to two copies, detected using the ddPCR SMN1 Copy Number Determination Kit. All error bars are generated by QuantaSoft™ Software and represent a 95% confidence interval.

Detection of SMN2 copy number. A 2-D fluorescence amplitude plot shows the wells of a sample duplexed with the SMN2 and reference assays. The black cluster on the plot represents the negative droplets, the green cluster represents the droplets that are positive for the reference only, the blue cluster within the red circle represents the droplets that are present for SMN2 only, and the orange cluster represents the droplets that are positive for both SMN2 and the reference target.

Accurate and reproducible copy number calls for SMN2. Copy number plot shows positive controls, ranging from two to four copies, detected using the ddPCR SMN2 Copy Number Determination Kit. All error bars are generated by QuantaSoft™ Software and represent a 95% confidence interval.