Become a Master of ddPCR
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Latest ddPCR Master Class videos | ddPCR Master Class videos | See What the Masters are Saying | Maximizing Your ddPCR Resources | The Knowledge Vault | Contact a ddPCR Expert |
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Digital PCR is becoming a common technique in the modern molecular biology lab. Although the technology and chemistry is simple compared to other techniques, a solid understanding of how and why things work under real conditions will help you quickly become proficient with digital PCR technology. Discover how to become a master in digital PCR by following the series of videos covering foundational and advanced topics.
Latest ddPCR Master Class Videos
These videos are the latest to be added to our Master Class training series.
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Mixing Triplex Multiplexing
Learn more about the multiplexing capability in your ddPCR assay with this short Advanced ddPCR Concepts session, which covers Triplex Multiplexing.
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Copy Number Variation in Digital PCR: Linkage Part 2
See examples of the counting process and how linkage levels are calculated.
ddPCR Master Class Videos
The following videos cover both foundational and advanced topics in ddPCR. Remember to visit us on the regular basis as new videos are added.
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Foundation Part 1: Counting in ddPCR
Have you ever wondered how exactly molecules are counted? Learn about the counting process and discover practical examples of how we count using ddPCR.
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Copy Number Variation
See how you can calculate CNVs using ddPCR, as well as quick tips to get the most out of your assays.
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Copy Number Variation in Digital PCR: Linkage Part 1
Learn how you can take advantage of this type of assay. Also, see examples of how Linkage is detected.
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Copy Number Variation in Digital PCR: Linkage Part 2
See examples of the counting process and how linkage levels are calculated.
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Amplitude Multiplexing
Are you interested in multiplexing more targets than the number of colors your instrument has? This short Advanced ddPCR Concepts session covers Amplitude Multiplexing, which allows the accurate quantification of four independent targets using two colors on your system. Dianna Maar, Ph.D., describes how to plan and execute these types of experiments.
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Radial Multiplexing
This short advanced ddPCR concepts session covers Radial Multiplexing, which allows the accurate quantification of five or more independent targets using two colors on your system. Dianna Maar, Ph.D., describes how these assays are designed and how to execute these types of experiments.
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Mixing Triplex Multiplexing
Learn more about the multiplexing capability in your ddPCR assay with this short Advanced ddPCR Concepts session, which covers Triplex Multiplexing.
See What the Masters are Saying
See how experts across the world are using digital PCR to solve real-world challenges.
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QX100 Droplet Digital (ddPCR) System at Sangamo BioSciences
See how researchers at Sangamo BioSciences use Bio-Rad's QX100 ddPCR System to quantify the level of HIV reservoir in treatment with their unique zinc-finger-based therapy.
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Using Droplet Digital PCR to Diagnose Leukemia
See how Prof. Alec Morley, a pioneer in digital PCR, describes his use of Droplet Digital PCR (ddPCR) technology to advance the diagnosis and treatment of leukemia.
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Using Droplet Digital PCR to Quantify Levels of HIV Reservoirs
How to use Bio-Rad's Droplet Digital PCR System to quantify the level of HIV reservoirs in patients treated with their unique zinc-finger-based HIV therapy.
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Using Droplet Digital PCR to Monitor Environmental Contaminants in Water
Monitor environmental contaminants in water using ddPCR to surveil genetic targets such as pathogens, invasive species, or other biological activity that requires the ability to detect small target concentrations within complex, nonuniform samples.
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Using Droplet Digital PCR to Quantify Early Rejection Biomarkers in Transplant Patients
See how researchers at Chronix Biomedical applied Bio-Rad's ddPCR technology to quantify graft-derived cell-free DNA (cfDNA) in recent liver transplant patients.
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Breaking Leukemia's Limits of Detection with Droplet Digital PCR
See how Prof. Alec Morley, a pioneer in digital PCR, describes his use of Droplet Digital PCR (ddPCR) technology to advance the diagnosis and treatment of leukemia.
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High-Quality Gene Expression from Precious Samples — A Simplified Workflow
Find out how Mark Kibschull of Lunenfeld-Tanenbaum Research Institute in Toronto determines pluripotency and differentiation of limited human embryonic stem cells using Bio-Rad's simplified workflow to allow for the gene expression analysis of hundreds of genes without the cumbersome step of isolating and purifying RNA.
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Discover Decisive Moments in Personalized Cancer Treatment
See how decisive moments like choosing lab technologies, getting a liquid biopsy, or collaborating on clinical decisions look like in practice.
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How Liquid Biopsies Help Understanding of Resistance to Chemotherapy
Listen to how Dr. Oxnard, Assistant Professor, Dana-Farber Cancer Institute, discuss his research in resistance development of non-small cell lung cancers using ddPCR technology.
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Liquid Biopsy and Droplet Digital PCR Technology
Learn how with plasma genotyping, also known as liquid biopsy, you can offer a noninvasive method that evaluates tumor-derived cell-free DNA in a patient's blood.
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Future Breast Cancer Treatment Decisions
See how liquid biopsy is beginning to bring the power of noninvasive, highly sensitive, near real-time monitoring of treatment response to solid tumors.
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Overcoming a Diagnostic Challenge in Melanoma — Decisive Moment
Learn how liquid biopsy using ddPCR technology can help distinguish tumor DNA from non-malignant activity that would have been difficult to distinguish and diagnose using a typical scan.
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Drop-Phase: Phasing Genomes with Droplet Digital PCR
What is Drop-Phase? A technique to determine whether genomic sequences are linked rapidly. We review the PLOS ONE paper that introduced this technique and speak with its lead author, Jack Regan.
Maximizing Your ddPCR Resources
These videos provide an overview of instrument operation.
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Digital PCR Using the Bio-Rad QX100 ddPCR System
See how the QX100 System provides an absolute measure of target DNA molecules with unrivaled performance in precision, accuracy, and sensitivity for quantitative PCR applications.
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QX200 AutoDG Droplet Digital PCR System
See how Bio-Rad's QX200 AutoDG Droplet Digital PCR System provides absolute quantification of target DNA or RNA molecules for EvaGreen Assays and probe-based ddPCR applications.
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Droplet Digital PCR Using the Bio-Rad QX200 and AutoDG ddPCR Systems
See how the third-generation QX200 AutoDG System now brings automation and scalability to digital PCR.
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Bio-Rad's QX ONE Droplet Digital PCR System Tour
This video showcases the features and workflow of QX ONE Droplet Digital PCR System.
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Droplet Digital PCR Using Bio-Rad's QX100 ddPCR System
Quantify target DNA with unrivaled precision by partitioning samples into 20,00 nanoliter-sized droplets with Bio-Rad's state-of-the-art Droplet Digital PCR System. Learn more.
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Automated Droplet Generator (AutoDG) Workflow Tips for Success
Meet the AutoDG System: This video provides information that can improve data of AutoDG experiments showing tips and tricks in the workflow.
The Knowledge Vault
Discover ddPCR presentations that provide guidance from design to results in various application areas.
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Droplet Digital PCR Tips and Tricks: ddPCR Assay Design
Learn the tips and tricks used in Droplet Digital PCR (ddPCR) assay design, common pitfalls and recommendations for designing an effective ddPCR assay.
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The Power of Partitioning: The World of Droplet Digital PCR Applications
See how Droplet Digital PCR (ddPCR) has transformed multiple fields of research, including infectious disease, cancer biomarker analysis, and genomic variation analysis.
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Using Droplet Digital PCR for Cancer and Liquid Biopsy Studies
See how Droplet Digital PCR (ddPCR) technology is being used to identify cancer subtypes, optimize drug treatment plans, monitor residual disease, and study tumor evolution.
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Droplet Digital PCR for Gene Copy Number Variation
Learn how copy number variations (CNVs), from single gene to segmental duplications, are increasingly recognized as critical features of the human genome.
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Droplet Digital PCR for Gene Expression and MicroRNA Analysis
Discover Droplet Digital PCR (ddPCR) technology and its application in gene expression analysis and microRNA analysis. Further learn various multiplexing approaches using EvaGreen Dye in ddPCR studies.
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Droplet Digital PCR Applications for Next Generation Sequencing (NGS)
See how Droplet Digital PCR (ddPCR) applications work for cross-validating NGS data with rare species as well as the quantification of NGS libraries to optimize sequencing performance.
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Ultra-Sensitive Quantification of Genome Editing Events by Droplet Digital PCR (ddPCR)
Learn how gene editing using ddPCR is useful for ultra-sensitive detection of edited alleles and offers a rapid, low-cost readout for technical optimization of genome editing protocols.
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Methylation-Specific Droplet Digital PCR to Study Immune Cell Subtypes
See how researchers at UCSF use Bio-Rad's Droplet Digital PCR (ddPCR) to investigate aberrant gene methylation in their studies of the molecular subgroups of glioma, cancer arising from glial cells.
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Droplet Digital Applications: Phasing of Genetic Variants for CFTR Haplotyping
Watch Dr. Jack Regan describe how the Droplet Digital PCR (ddPCR) System can be used to determine if genomic sequences are physically linked, enabling us to determine disease carrier status, understand differences in allele-specific expression, and clarify the genome.
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Precision Molecular Profiling of Cancer Using Droplet Digital PCR
Learn how Droplet Digital PCR (ddPCR) technology is being used to identify cancer subtypes, optimize drug treatment plans, monitor residual disease, and study tumor evolution.
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Can Droplet Digital PCR Detect 22q11.2 Deletion Syndrome?
Hear how researchers at UC Davis have applied Droplet Digital PCR (ddPCR) technology to determine copy number variation (CNV) and characterize the deletion end points of the region of chromosome 22 that is deleted in 22q11DS.
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Counting with Droplet Digital PCR – More Applications than Anyone Ever Dreamed Of
See how the adoption of ddPCR for liquid biopsy in cancer therapy monitoring, assisting genome editing of stem cells for disease modeling and therapy, in vivo count monitoring of therapeutically engineered immune cells in HIV eradication strategies, and identification of under-recognized somatic mosaicisms.
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Using Droplet Digital PCR to Study Stem Cell Genomes at Stanford University
In this webinar, see how Prof. Alexander Urban at the Stanford School of Medicine discusses how the his lab used the QX200 ddPCR System to investigate copy number variation in induced pluripotent stem cells (iPSCs), an offshoot their studies of normal and abnormal brain development and function related to autism.
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Digital Quantification of Potential Therapeutic Target RNAs
See how Dr. David Dodd describes the application of ddPCR in his research studies has allowed for antisense oligonucleotides and duplex RNAs to control gene expression in human cancer cells.
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Precise Counting of Targeted Nucleic Acids Has Never Been Easier
Watch this overview of ddPCR technology from technical and workflow-related perspectives and reviews key applications such as CNV analysis, rare mutation detection and standard-free absolute quantification of nucleic acids.
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Applying Droplet Digital PCR to the Study of Environmental Samples
See how the use of ddPCR with environmental samples on the QX200 Droplet Digital PCR (ddPCR) System provides absolute quantification.
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In Vivo Monitoring of CAR-T Cells Using Sensitive Droplet Digital PCR
View how Droplet Digital PCR is the perfect tool to detect gene-modified cells in different body fluids.
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Webinar: A New Approach to Monitoring Residual Disease in Chronic Myeloid Leukaemia
Find out the results of validation tests of the Droplet Digital PCR technology and the CE-IVD QXDx BCR-ABL% IS Kit.
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Bio-Rad's Expert Panel Discussion: COVID-19 with Droplet Digital PCR and Antibody Testing
Get an overview of the approaches that Bio-Rad is taking in the battle against COVID-19: Detection and diagnosis with Droplet Digital PCR.
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Bio-Rad's Expert Panel Discussion: Relevance of COVID-19 Testing with Droplet Digital PCR
Learn more about the comparison of ddPCR to RT-PCR technologies for SARS-CoV-2.
All videos are presented in English unless noted otherwise.
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